Part:BBa_K2644003
ArsR
Arsenic binding repressor protein--ArsR and ArsR promoter. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This part consists of the ArsR repressor binding site and the ArsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter.
Results
We used this part to build a new arsenic induction loop through it. Therefore, we first added 10μl ddH2O to the corresponding hole, and then took PCR was performed at 1 μl, and the corresponding DNA fragment was obtained.
Figure1. The result of nucleic acid gel electrophoresis of Bba-J33201 after PCR. Lane M, Marker. Lane 1-6，Bba-J33201
Then we performed PCR on the promoter fragment and ArsR fragment in this fragment, and the results are as follows.
Figure2. The result of nucleic acid gel electrophoresis of Ars Promoter after PCR. lane M, Marker. Lane 1-4, Ars Promoter.
Figure 3. The result of nucleic acid gel electrophoresis of ArsR Protein after PCR. Lane M, Marker. Line1, ArsR Protein.
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